4.7 Article

In Vivo Silencing of Genes Coding for dTip60 Chromatin Remodeling Complex Subunits Affects Polytene Chromosome Organization and Proper Development in Drosophila melanogaster

Journal

Publisher

MDPI
DOI: 10.3390/ijms22094525

Keywords

Drosophila melanogaster; polytene chromosomes; chromatin remodeling; position effect variegation; epigenetic silencing

Funding

  1. Pasteur Institute of Italy
  2. Fondazione Cenci-Bolognetti
  3. Sapienza University of Rome, Progetti di Ricerca di Ateneo [RM120172B851A176]

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The chromatin organization in Drosophila melanogaster is regulated by Tip60 chromatin remodeling complex (dTip60), DOM-A.C and DOM-B.C complexes, which share most subunits. This study combined genetics and cell biology to investigate the biological roles of these subunits in Drosophila melanogaster development.
Chromatin organization is developmentally regulated by epigenetic changes mediated by histone-modifying enzymes and chromatin remodeling complexes. In Drosophila melanogaster, the Tip60 chromatin remodeling complex (dTip60) play roles in chromatin regulation, which are shared by evolutionarily-related complexes identified in animal and plants. Recently, it was found that most subunits previously assigned to the dTip60 complex are shared by two related complexes, DOM-A.C and DOM-B.C, defined by DOM-A and DOM-B isoforms, respectively. In this work, we combined classical genetics, cell biology, and reverse genetics approaches to further investigate the biological roles played during Drosophila melanogaster development by a number of subunits originally assigned to the dTip60 complex.

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