Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/ijms22094568
Keywords
age related macular degeneration (AMD); diabetic macular edema (DME); cytopathic hypoxia; retinal pigment epithelial cells (RPE); ARPE-19; CoCl2; seahorse; ECIS modeling; R-b resistance; alpha resistance; impedance; capacitance; barrier integrity
Funding
- American Heart Association Grant [18CDA34080403]
- NIH [P30EY004068]
- NIH/NEI [EY023992]
- Research to Prevent Blindness
Ask authors/readers for more resources
This study investigated the impact of cytopathic hypoxia on the barrier functionality of RPE cells through ECIS system and seahorse technology, revealing a dose-dependent reduction in total impedance of ARPE-19 cells by CoCl2 treatment. Furthermore, CoCl2 significantly impaired mitochondrial function and decreased expression of ZO-1, a major paracellular tight junction protein, in ARPE-19 cells.
Disruption of retinal pigment epithelial (RPE barrier integrity is a hallmark feature of various retinal blinding diseases, including diabetic macular edema and age-related macular degeneration, but the underlying causes and pathophysiology are not completely well-defined. One of the most conserved phenomena in biology is the progressive decline in mitochondrial function with aging leading to cytopathic hypoxia, where cells are unable to use oxygen for energy production. Therefore, this study aimed to thoroughly investigate the role of cytopathic hypoxia in compromising the barrier functionality of RPE cells. We used Electric Cell-Substrate Impedance Sensing (ECIS) system to monitor precisely in real time the barrier integrity of RPE cell line (ARPE-19) after treatment with various concentrations of cytopathic hypoxia-inducing agent, Cobalt(II) chloride (CoCl2). We further investigated how the resistance across ARPE-19 cells changes across three separate parameters: R-b (the electrical resistance between ARPE-19 cells), alpha (the resistance between the ARPE-19 and its substrate), and C-m (the capacitance of the ARPE-19 cell membrane). The viability of the ARPE-19 cells and mitochondrial bioenergetics were quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and seahorse technology, respectively. ECIS measurement showed that CoCl2 reduced the total impedance of ARPE-19 cells in a dose dependent manner across all tested frequencies. Specifically, the ECIS program's modelling demonstrated that CoCl2 affected R-b as it begins to drastically decrease earlier than alpha or C-m, although ARPE-19 cells' viability was not compromised. Using seahorse technology, all three concentrations of CoCl2 significantly impaired basal, maximal, and ATP-linked respirations of ARPE-19 cells but did not affect proton leak and non-mitochondrial bioenergetic. Concordantly, the expression of a major paracellular tight junction protein (ZO-1) was reduced significantly with CoCl(2-)treatment in a dose-dependent manner. Our data demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells' viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available