Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ijms22084007
Keywords
microscopy; fluorescence microscopy; FISH; DAPI; Desulfovibrio; anaerobic microorganisms; habitats; SRB; SRP; SRM; sulfate reduction; identification; gut microbiota; IBD
Funding
- Grant Agency of Masaryk University [MUNI/A/1425/2020]
- University of Vienna
Ask authors/readers for more resources
This paper explores microscopic methods for identifying sulfate-reducing bacteria (SRB), discussing their presence in various habitats and the techniques used for their detection. It also highlights the economic and health implications of SRB in industries and human microbiota, emphasizing the importance of methods for detecting these microorganisms, including molecular and fluorescence techniques.
This paper is devoted to microscopic methods for the identification of sulfate-reducing bacteria (SRB). In this context, it describes various habitats, morphology and techniques used for the detection and identification of this very heterogeneous group of anaerobic microorganisms. SRB are present in almost every habitat on Earth, including freshwater and marine water, soils, sediments or animals. In the oil, water and gas industries, they can cause considerable economic losses due to their hydrogen sulfide production; in periodontal lesions and the colon of humans, they can cause health complications. Although the role of these bacteria in inflammatory bowel diseases is not entirely known yet, their presence is increased in patients and produced hydrogen sulfide has a cytotoxic effect. For these reasons, methods for the detection of these microorganisms were described. Apart from selected molecular techniques, including metagenomics, fluorescence microscopy was one of the applied methods. Especially fluorescence in situ hybridization (FISH) in various modifications was described. This method enables visual identification of SRB, determining their abundance and spatial distribution in environmental biofilms and gut samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available