Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms22073459
Keywords
self-assembly; peptide amphiphile; enzymatic reaction; pH-responsiveness; post-modification
Funding
- Japan Association for Chemical Innovation
- Iketani Science and Technology Foundation
- Shiseido Female Researcher Science Grant
- Kyushu University Program for Leading Graduate Schools, Advanced Graduate da Vinci Course on Molecular Systems for Devices
- Kyushu University Program for Leading Graduate Schools, Advanced Graduate Course on Molecular Systems for Devices
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This study investigated the self-assembly and enzymatic post-modification of short aromatic peptide amphiphiles, showing that these molecules can form fibrous structures and their assembly is influenced by solution pH. Moreover, enzymatic reactions showed different rates at varying pH, indicating pH dependency in the enzyme-catalyzed post-modification process.
Supramolecular fibrous materials in biological systems play important structural and functional roles, and therefore, there is a growing interest in synthetic materials that mimic such fibrils, especially those bearing enzymatic reactivity. In this study, we investigated the self-assembly and enzymatic post-modification of short aromatic peptide amphiphiles (PAs), Fmoc-L(n)QG (n = 2 or 3), which contain an LQG recognition unit for microbial transglutaminase (MTG). These aromatic PAs self-assemble into fibrous structures via pi-pi stacking interactions between the Fmoc groups and hydrogen bonds between the peptides. The intermolecular interactions and morphologies of the assemblies were influenced by the solution pH because of the change in the ionization states of the C-terminal carboxy group of the peptides. Moreover, MTG-catalyzed post-modification of a small fluorescent molecule bearing an amine group also showed pH dependency, where the enzymatic reaction rate was increased at higher pH, which may be because of the higher nucleophilicity of the amine group and the electrostatic interaction between MTG and the self-assembled Fmoc-L(n)QG. Finally, the accumulation of the fluorescent molecule on these assembled materials was directly observed by confocal fluorescence images. Our study provides a method to accumulate functional molecules on supramolecular structures enzymatically with the morphology control.
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