Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms22073314
Keywords
epigenetics; herbicide resistance; chromatin mutants; ROS1; imazethapyr; glyphosate; 2; 4-D
Funding
- CAPES fellowship [BEX 10896/14-7]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq [437317/2018-8]
- German Research Foundation (DFG) within the Science Priority Program 1529 Adaptomics [AP1859-2]
- Purkyne fellowship
- European Regional Development Fund project 'Plants as a tool for sustainable global development' [CZ.02.1.01/0.0/0.0/16_019/0000827]
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The study reveals that herbicide treatment impacts transcriptional gene silencing pathways, and misregulation of these pathways results in increased sensitivity of Arabidopsis plants to herbicide treatment.
Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in REPRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and ros1 plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.
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