Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ijms22063112
Keywords
fluconazole; hydrogels; drug carriers; fungal infections; chronic wounds
Funding
- National Science Centre in Poland (NCN Poland) [UMO-2015/19/B/ST5/01330, UMO-2016/21/B/NZ6/01157]
- National Science Centre Poland (NCN) [UMO-2018/28/T/ST5/00326]
Ask authors/readers for more resources
New fluconazole-loaded hydrogels incorporating nanohydroxyapatite were prepared and characterized using XRD, SEM-EDS, and ATR-FTIR. The antifungal activity of fluconazole was tested against various Candida, Cryptococcus, and Rhodotorula species. The hydrogels were found to be excellent drug carriers and effective in preventing fungal infections in chronic wounds.
New fluconazole-loaded, 6-Anhydro-alpha-l-Galacto-beta-d-Galactan hydrogels incorporated with nanohydroxyapatite were prepared and their physicochemical features (XRD, X-ray Diffraction; SEM-EDS, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy; ATR-FTIR, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy), fluconazole release profiles and enzymatic degradation were determined. Antifungal activity of pure fluconazole was tested using Candida species (C. albicans, C. tropicalis, C. glabarata), Cryptococcus species (C. neoformans, C. gatti) and Rhodotorula species (R. mucilaginosa, R. rubra) reference strains and clinical isolates. Standard microdilution method was applied, and fluconazole concentrations of 2-250 mu g/mL were tested. Moreover, biofilm production ability of tested isolates was tested on the polystyrene surface at 28 and 37 +/- 0.5 degrees C and measured after crystal violet staining. Strains with the highest biofilm production ability were chosen for further analysis. Confocal microscopy photographs were taken after live/dead staining of fungal suspensions incubated with tested hydrogels (with and without fluconazole). Performed analyses confirmed that polymeric hydrogels are excellent drug carriers and, when fluconazole-loaded, they may be applied as the prevention of chronic wounds fungal infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available