Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ijms22084144
Keywords
β -galactosidase; Antarctica; lactose; cold-adapted bacteria; extremozymes
Funding
- INSTITUTO ANTARTICO CHILENO (INACH) [INACH RT_14-12, INACH DG_01-19]
- UFRO/UANDES [INI4]
- UNIVERSIDAD DE LA FRONTERA [DI19-0079, DI20-2018]
- NETWORK FOR EXTREME ENVIRONMENTS RESEARCH (NEXER) [NXR17-0003]
- CONICYT [CONICYT-PFCHA/Doctorado Nacional/2017-21170263, CONICYT-PFCHA/Doctorado Nacional/2017-21171392, CONICYT-PFCHA/Doctorado Nacional/2017-21170265]
- National Agency for Research and Development (ANID Chile) (Fondecyt Iniciacion) [11170081]
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This study identified a new cold-active beta-galactosidase producer from Antarctic bacterial strains, demonstrating unique enzymatic activity in cold conditions. Additionally, three beta-galactosidase genes were found in Rahnella inusitata genome, showing potential for commercial applications in the dairy industry.
There has been a recent increase in the exploration of cold-active beta-galactosidases, as it offers new alternatives for the dairy industry, mainly in response to the current needs of lactose-intolerant consumers. Since extremophilic microbial compounds might have unique physical and chemical properties, this research aimed to study the capacity of Antarctic bacterial strains to produce cold-active beta-galactosidases. A screening revealed 81 out of 304 strains with beta-galactosidase activity. The strain Se8.10.12 showed the highest enzymatic activity. Morphological, biochemical, and molecular characterization based on whole-genome sequencing confirmed it as the first Rahnella inusitata isolate from the Antarctic, which retained 41-62% of its beta-galactosidase activity in the cold (4 degrees C-15 degrees C). Three beta-galactosidases genes were found in the R. inusitata genome, which belong to the glycoside hydrolase families GH2 (LacZ and EbgA) and GH42 (BglY). Based on molecular docking, some of these enzymes exhibited higher lactose predicted affinity than the commercial control enzyme from Aspergillus oryzae. Hence, this work reports a new Rahnella inusitata strain from the Antarctic continent as a prominent cold-active beta-galactosidase producer.
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