4.7 Article

Metabolic engineering of Methylorubrum extorquens AM1 for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production using formate

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 177, Issue -, Pages 284-293

Publisher

ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.02.092

Keywords

PHBV copolymer; Formate; SCFAs; Methylorubrum extorquens AM1; Metabolic engineering

Funding

  1. National Research Foundation of Korea (NRF) - Ministry of Science and ICT, South Korea [2015M3D3A1A01064919, 2012M1A2A2026560]

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By genetic engineering of M. extorquens and addition of short-chain fatty acids, a PHBV copolymer with a high proportion of 3HV can be synthesized using formate as the carbon source.
Formate is a promising environmentally friendly and sustainable feedstock synthesized from syngas or carbon dioxide. Methylorubrum extorquens is a type II methylotroph that can use formate as a carbon source. It accumulates polyhydroxyalkanoates (PHAs) inside the cell, mainly producing poly-3-hydroxybutyrate (PHB), a degradable biopolymer. Owing to its high melting point and stiff nature, however, mechanical property improvement is warranted in the form of copolymerization. To produce the PHA copolymer, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), the endogenous gene phaC was deleted and the pathway genes bktB, phaJ1, and phaC2, with broader substrate specificities, were heterologously expressed. To improve the incorporation of 3hydroxyvalerate (3HV), the expression level of bktB was improved by untranslated region (UTR) engineering, and the endogenous gene phaA was deleted. The engineered M. extorquens produced PHBV with 8.9% 3HV using formate as the sole carbon source. In addition, when propionate and butyrate were supplemented, PHBVs with 3HV portions of up to 70.6% were produced. This study shows that a PHBV copolymer with a high proportion of 3HV can be synthesized using formate, a C1 carbon source, through metabolic engineering and supplementation with short-chain fatty acids. (c) 2021 Elsevier B.V. All rights reserved.

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