Journal
INFECTION AND IMMUNITY
Volume 89, Issue 6, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00808-20
Keywords
Brucella; cysteine; epithelial cells; glutathione; intracellular pathogen; macrophages; metabolism; sulfur
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Funding
- NIH [R35 GM131762, T32 GM007197]
- Gallo Global Health Fellowship Program
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Brucella ovis is an intracellular pathogen with tropism for the male genital tract in ovine. Studies of stationary phase may inform understanding of Brucella infection biology, but genes and pathways important in Brucella stationary-phase physiology are poorly defined. Research has found that cysteine synthesis is essential for peroxide stress survival and fitness of B. ovis in the intracellular niche.
Brucella ovis is an ovine intracellular pathogen with tropism for the male genital tract. To establish and maintain infection, B. ovis must survive stressful conditions inside host cells, including low pH, nutrient limitation, and reactive oxygen species. The same conditions are often encountered in axenic cultures during stationary phase. Studies of stationary phase may thus inform our understanding of Brucella infection biology, yet the genes and pathways that are important in Brucella stationary-phase physiology remain poorly defined. We measured fitness of a barcoded pool of B. ovis Tn-himar mutants as a function of growth phase and identified cysE as a determinant of fitness in stationary phase. CysE catalyzes the first step in cysteine biosynthesis from serine, and we provide genetic evidence that two related enzymes, CysK1 and CysK2, function redundantly to catalyze cysteine synthesis at steps downstream of CysE. Deleting cysE (Delta cysE) or both cysK1 and cysK2 (Delta cysK1 Delta cysK2) results in premature entry into stationary phase, reduced culture yield, and sensitivity to exogenous hydrogen peroxide. These phenotypes can be chemically complemented by cysteine or glutathione. Delta cysE and Delta cysK1 Delta cysK2 strains have no defect in host cell entry in vitro but have significantly diminished intracellular fitness between 2 and 24 h postinfection. Our study has uncovered unexpected redundancy at the CysK step of cysteine biosynthesis in B. ovis and demonstrates that cysteine anabolism is a determinant of peroxide stress survival and fitness in the intracellular niche.
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