Journal
IMMUNITY
Volume 54, Issue 5, Pages 962-+Publisher
CELL PRESS
DOI: 10.1016/j.immuni.2021.03.011
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Funding
- Chinese Ministry of Science and Technology [2019YFA0508500, 2020YFA 0707800]
- National Natural Science Foundation of China [31830022, 81621001]
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The biosynthesis of sulfated glycosaminoglycans (sGAGs) in the Golgi apparatus is crucial for the polymerization and activation of the STING receptor. sGAGs promote STING polymerization through luminal, positively charged, polar residues, and the chain length and sulfation of sGAGs directly affect STING activation. The translocation from the endoplasmic reticulum to the Golgi apparatus is necessary for STING activation.
Activation of the cyclic guanosine monophosphate (GMP)-AMP (cGAMP) sensor STING requires its translocation from the endoplasmic reticulum to the Golgi apparatus and subsequent polymerization. Using a genome-wide CRISPR-Cas9 screen to define factors critical for STING activation in cells, we identified proteins critical for biosynthesis of sulfated glycosaminoglycans (sGAGs) in the Golgi apparatus. Binding of sGAGs promoted STING polymerization through luminal, positively charged, polar residues. These residues are evolutionarily conserved, and selective mutation of specific residues inhibited STING activation. Purified or chemically synthesized sGAGs induced STING polymerization and activation of the kinase TBK1. The chain length and O-linked sulfation of sGAGs directly affected the level of STING polymerization and, therefore, its activation. Reducing the expression of Slc35b2 to inhibit GAG sulfation in mice impaired responses to vaccinia virus infection. Thus, sGAGs in the Golgi apparatus are necessary and sufficient to drive STING polymerization, providing a mechanistic understanding of the requirement for endoplasmic reticulum (ER)-toGolgi apparatus translocation for STING activation.
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