Journal
GENOME BIOLOGY AND EVOLUTION
Volume 13, Issue 5, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evab087
Keywords
SARS-CoV-2; COVID-19; mutation; selection; sequencing; viral genomics
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Funding
- European Molecular Biology Laboratory (EMBL) [R35GM128932]
- Alfred P. Sloan foundation fellowship
- Australian Research Council [DP200103151]
- Chan-Zuckerberg Initiative grant
- Australian Research Council [DP200103151] Funding Source: Australian Research Council
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The COVID-19 pandemic has prompted an unprecedented response from the sequencing community, leading to a study of mutation rates and selective pressures using sequence data from over 140,000 SARS-CoV-2 genomes. Two specific mutation rates, G -> U and C -> U, were found to be significantly higher than others, possibly attributed to APOBEC and ROS activity. Genomic context does have an effect on mutation rates, but its impact is limited.
The COVID-19 pandemic has seen an unprecedented response from the sequencing community. Leveraging the sequence data from more than 140,000 SARS-CoV-2 genomes, we study mutation rates and selective pressures affecting the virus. Understanding the processes and effects of mutation and selection has profound implications for the study of viral evolution, for vaccine design, and for the tracking of viral spread. We highlight and address some common genome sequence analysis pitfalls that can lead to inaccurate inference of mutation rates and selection, such as ignoring skews in the genetic code, not accounting for recurrent mutations, and assuming evolutionary equilibrium. We find that two particular mutation rates, G -> U and C -> U, are similarly elevated and considerably higher than all other mutation rates, causing the majority of mutations in the SARS-CoV-2 genome, and are possibly the result of APOBEC and ROS activity. These mutations also tend to occur many times at the same genome positions along the global SARS-CoV-2 phylogeny (i.e., they are very homoplasic). We observe an effect of genomic context on mutation rates, but the effect of the context is overall limited. Although previous studies have suggested selection acting to decrease U content at synonymous sites, we bring forward evidence suggesting the opposite.
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