Establishment of lncRNA-mRNA network in bovine oocyte between germinal vesicle and metaphase II stage

Long non-coding RNA (lncRNA), a type of non-protein coding transcripts with lengths exceeding 200 nucleotides, is reported to be widely involved in many cellular and developmental processes. However, few roles of lncRNA in oocyte development have been defined. In this study, to uncover the effect of lncRNA during oocyte maturation, bovine germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes underwent RNA sequencing. Results revealed a wealth of candidate lncRNAs, which might participate in the biological processes of stage-specific oocytes. Furthermore, their trans- and cis-regulatory effects were investigated in-depth by using bioinformatic software. Functional enrichment analysis of target genes showed that these lncRNAs were likely involved in the regulation of many key signaling pathways during bovine oocyte maturation from GV to MII stage, as well as multiple lncRNA-mRNA networks. One novel lncRNA (MSTRG.19140) was particularly interesting, as it appeared to mediate the regulation of oocyte meiotic resumption, progesterone-mediated oocyte maturation, and cell cycle. Therefore, this study enhanced insights into the regulation of molecular mechanisms of bovine oocyte maturation from a lncRNA-mRNA network perspective.

Automated summary

This study investigated the role of long non-coding RNA (lncRNA) in bovine oocyte development through RNA sequencing, identifying candidate lncRNAs and exploring their regulatory effects using bioinformatic software. Functional enrichment analysis revealed the involvement of these lncRNAs in regulating key signaling pathways and lncRNA-mRNA networks during bovine oocyte maturation. Additionally, a novel lncRNA (MSTRG.19140) was found to play a role in regulating oocyte meiotic resumption, progesterone-mediated oocyte maturation, and cell cycle.