4.7 Article

Absence of Toxoplasma gondii in 100% Iberian products from experimentally infected pigs cured following a specific traditional process

Journal

FOOD MICROBIOLOGY
Volume 95, Issue -, Pages -

Publisher

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2020.103665

Keywords

Toxoplasma gondii; Iberian pig; Magnetic capture; Real-time PCR; Ham curing processes; Bioassay

Funding

  1. Spanish Ministry of Science [RTC-2017-6263-2]

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The study focused on detecting Toxoplasma gondii in cured ham from 100% Iberian sows raised on acorn feed in Spain, showing that the specific curing process used in making the ham effectively eliminates the parasite, making it a safe product for consumers.
Infection with Toxoplasma gondii in humans has usually been related to the consumption of raw, undercooked or cured meat. Our study is based on the detection of T. gondii in cured legs and shoulders made from 100% Iberian sows fed mainly with acorn and raised as outdoor livestock in Aracena (Spain), which having been elaborated following a specific curing process (time period and location). An outdoor farm with a total of 636 animals was studied, showing a seroprevalence of 10% for the parasite T. gondii. Twenty individuals were chosen to be experimentally infected and slaughtered 60 days post-infection. Their legs and shoulders were processed to make 100% Iberian ham legs and shoulders. The meat ready to be eaten was analyzed by quantification and viability assays using magnetic capture real-time qPCR and bioassay techniques proving that this specific traditional Cinco Jotas curing process 100% Iberian ham is strong enough to eliminate the parasite T. gondii, resulting in a safe product for consumers.

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