Journal
FOOD CHEMISTRY
Volume 343, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128533
Keywords
Food allergens; Mass spectrometry; Peptide markers; ThRAll; Discovery analysis; Incurred matrix
Funding
- European Food Safety Authority (EFSA) [GP/EFSA/AFSCO/2017/03]
- UK Food Standards Agency [FS101209, FS101206]
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The study focuses on discovering the most reliable peptide markers in multiple food matrices, laying the foundation for the development of a mass spectrometry method for multiple allergen detection.
Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.
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