4.7 Article

Identification of milk adulteration by a sensor array based on cationic polymer induced aggregation of a perylene probe

Journal

FOOD CHEMISTRY
Volume 343, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128492

Keywords

Milk adulteration; Sensor array; Perylene probe; Cationic polymer; Milk protein; Fluorescence

Funding

  1. National Natural Science Foundation of China [51761145102, 21874128, 31772058]
  2. Natural Science Foundation of Jilin Province [20190201069JC, 20190302088GX, 20200201092JC]

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A novel fluorescence sensor array has been developed through cationic polymer-induced self-assembly to discriminate main proteins in milk and detect milk adulteration with high accuracy. The sensor array shows great potential for quantitative detection of milk adulteration.
A novel fluorescence sensor array based on cationic polymer induced self-assembly of a perylene probe is developed. Cationic polymer induced aggregation of the carboxyl modified negatively charged perylene probe, and resulted in large quenching of monomer emission and generation of excimer emission. Upon the addition of negatively charged protein, monomer fluorescence restored with a decrease in excimer fluorescence. Based on these observations, we developed a six-channel sensor array to discriminate five main proteins in milk. In addition, we successfully identified pure milk out of different drinks using the developed sensor array since different drinks contained distinct species and contents of proteins. Furthermore, the sensor array exhibited excellent performance to discriminate milk adulterated by different concentrations of adulterants with 100% accuracy of cross validation. The analysis results also presented excellent linear correlation of adulterants contents and thus the developed sensor array shows great potential for quantitative detection of milk adulteration.

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