Journal
FOOD CHEMISTRY
Volume 344, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128656
Keywords
Phosphoproteins; Quartz crystal microbalance sensor; Aminated nano-titanium dioxide; Ultrafast determination; Food
Funding
- National Key R&D Program of China [2016YFD0401202]
- State Key Laboratory of Food Nutrition and Safety [SKLFNS-KF-202010]
- Special Project of Tianjin Innovation Platform [17PTGCCX00230]
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The novel quartz crystal microbalance sensor utilizes NH2-TiO2 for efficient detection of trace phosphoproteins, showing a low detection limit and linear frequency shift characteristics. Compared to traditional systems, the analysis process of this sensor is simpler and faster, completing within 5 minutes.
A novel quartz crystal microbalance (QCM) sensor for trace-phosphoprotein ultrafast detection was constructed based on the bridge interactions between the NH2-TiO2 sites enriched on Au-electrode and phosphate groups. Herein, 11-mercaptoundecanoic acid (MUA) modified by Au-S bond acted as carrier for immobilizing NH2-TiO2. Functionalized NH2-TiO2 to absorb phosphoproteins. Under the optimal conditions, the proposed sensor showed a linear frequency shift to the concentration of alpha-casein ranging from 1.0 x 10(-3) to 1.0 mg mL(-1) with a low detection limit of 5.3 x 10(-6) mg mL(-1) (S/N = 3), and the limit of quantitation was 0.001 mg mL(-1). Compared with traditional Ti4+-IMAC/MOAC-system, the analysis process of NH2-TiO2/MUA/AuE-QCM sensor was simpler and faster which could complete within 5 min. Additionally, the constructed biosensor was successfully used for the non-fat milk and chicken egg white. This proposed sensor presents a great prospective strategy for the evaluation of the nutrition in different foods.
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