The SLIM1 transcription factor is required for arsenic resistance in Arabidopsis thaliana

The transcriptional regulators of arsenic-induced gene expression remain largely unknown. Sulfur assimilation is tightly linked with arsenic detoxification. Here, we report that mutant alleles in the SLIM1 transcription factor are substantially more sensitive to arsenic than cadmium. Arsenic treatment caused high levels of oxidative stress in the slim1 mutants, and slim1 alleles were impaired in both thiol accumulation and sulfate accumulation. We further found enhanced arsenic accumulation in roots of slim1 mutants. Transcriptome analyses indicate an important role for SLIM1 in arsenic-induced tolerance mechanisms. The present study identifies the SLIM1 transcription factor as an essential component in arsenic tolerance and arsenic-induced gene expression. Our results suggest that the severe arsenic sensitivity of the slim1 mutants is caused by altered redox status.

Automated summary

The SLIM1 transcription factor plays a crucial role in arsenic-induced gene expression and tolerance mechanisms. Mutant alleles in SLIM1 exhibit higher sensitivity to arsenic than cadmium, leading to increased oxidative stress and impaired sulfur assimilation. Enhanced arsenic accumulation in roots of slim1 mutants suggests altered redox status as a possible cause for their severe arsenic sensitivity.