Normoxic induction of HIF-1α by adenosine-A2BR signaling in epicardial stromal cells formed after myocardial infarction

Myocardial infarction (MI) activates the epicardium to form epicardial stromal cells (EpiSC) that reside in the epicardial hypoxic microenvironment. Paracrine factors secreted by EpiSC were shown to modulate the injury response of the post-MI heart and improve cardiac function. We have previously reported that the expression of the angiogenic cytokines vascular endothelial growth factor A (VEGFA) and IL-6 is strongly upregulated in EpiSC by adenosine acting via the A(2B) receptor (A(2B)R). Since tissue hypoxia is well known to be a potent stimulus for the generation of extracellular adenosine, the present study explored the crosstalk of A(2B)R activation and hypoxia-hypoxia-inducible factor 1 alpha (HIF-1 alpha) signaling in cultured EpiSC, isolated from rat hearts 5 days after MI. We found substantial nuclear accumulation of HIF-1 alpha after A(2B)R activation even in the absence of hypoxia. This normoxic HIF-1 alpha induction was PKC-dependent and involved upregulation of HIF-1 alpha mRNA expression. While the influence of hypoxia on adenosine generation and A(2B)R signaling was only minor, hypoxia and A(2B)R activation cumulatively increased VEGFA expression. Normoxic A(2B)R activation triggered an HIF-1 alpha-associated cell-protective metabolic switch and reduced oxygen consumption. HIF-1 alpha targets and negative regulators PHD2 and PHD3 were only weakly induced by A(2B)R signaling, which may result in a sustained HIF-1 alpha activity. The A(2B)R-mediated normoxic HIF-1 alpha induction was also observed in cardiac fibroblasts from healthy mouse hearts, suggesting that this mechanism is also functional in other A(2B)R-expressing cell types. Altogether, we identified A(2B)R-mediated HIF-1 alpha induction as novel aspect in the HIF-1 alpha-adenosine crosstalk, which modulates EpiSC activity and can amplify HIF-1 alpha-mediated cardioprotection.

Automated summary

This study identified A(2B)R-mediated HIF-1 alpha induction as a novel aspect in the HIF-1 alpha-adenosine crosstalk, which modulates EpiSC activity and can potentially enhance HIF-1 alpha-mediated cardioprotection.