Enhanced phenotype of calcipotriol-induced atopic dermatitis in filaggrin-deficient mice

Impaired function of filaggrin (FLG) is a major predisposing factor for atopic dermatitis (AD). Several studies on FLG-deficient (Flg(-/-)) mice have indicated an essential role for FLG in the skin barrier and the development of AD, but none of the studies have described the characteristics on Flg(-/-) mice with calcipotriol (CPT)-induced atopic dermatitis, which restricts the comprehensive understanding of functions of FLG. The present study sought to generate Flg(-/-) mice and applied CPT to produce AD-like dermatitis for in vivo analysis of the FLG functions. CPT was applied on the skin of Flg(-/-) mice to establish the AD-like dermatitis mouse model. The lesion inflammation was evaluated by gross ear thickness, histopathology, immunofluorescence, and cytokine production. Also, mucopolysaccharide polysulfate (MPS) and ceramide were used to observe the therapeutic function in this model. The results showed that the inflammation of CPT-induced dermatitis in Flg(-/-) mice was more severer than that of wild-type (WT) mice, as evident by the increased level of gross appearance, ear thickness, inflammatory cell infiltration (mast cells and CD3(+) T cells), and inflammatory cytokine expression (interleukin (IL)-4, IL-6, IL-13, and thymic stromal lymphopoietin (TSLP)). The emollients MPS and ceramide partially restored the epidermal function and alleviated the skin inflammation in Flg(-/-) mice with CPT-induced AD--like dermatitis. The current study demonstrated that skin barrier protein FLG is critical in the pathogenesis of AD. Also, the AD mouse model induced by CPT in Flg(-/-) mice could be utilized to search for drug targets in AD.

Automated summary

This study demonstrated the critical role of skin barrier protein FLG in the pathogenesis of atopic dermatitis, with CPT-induced dermatitis being more severe in Flg(-/-) mice compared to wild-type mice. Additionally, emollients MPS and ceramide partially restored epidermal function and alleviated skin inflammation in the Flg(-/-) mouse model of AD induced by CPT.