Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 145, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2021.109749
Keywords
Enzyme immobilization; Phasins PhaPs; Polyhydroxyalkanoate PHAs; Electrospinning; Nanofibers; Lipase
Categories
Funding
- Ministry of SMEs and Startups, Korea [S2591980]
- National Research Foundation of Korea [2016M1A5A 1027465, 2020R1A2C2013114, 2020R1A5A8017671]
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [S2591980] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2020R1A2C2013114, 2020R1A5A8017671] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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By using electrospun polyhydroxybutyrate nanofibers as an alternative support for enzyme immobilization, it is possible to anchor enzymes more efficiently and improve their stability and activity compared to conventional immobilization supports. This approach combines the advantageous features of nanofibrous material and specificity of biomolecular interaction, and can be widely adopted in the development of various enzymatic processes.
Enzyme immobilization provides substantial advantages in terms of improving the efficiency of enzymatic process as well as enhancing the reusability of enzymes. Phasins (PhaPs) are naturally occurring polyhydroxyalkanoate (PHA)-binding proteins, and thus can potentially be used as a fusion partner for oriented immobilization of enzymes onto PHA supports. However, presently available granular PHA supports have low surface-area-to-volume ratio and limited configurational flexibility of enzymatic reactions. In this study, we explored the use of electrospun polyhydroxybutyrate (PHB) nanofibers as an alternative support for high density immobilization of a PhaP-fused lipase. As envisioned, the electrospun PHB nanofibers could anchor 120-fold more enzyme than PHB granules of the same weight. Furthermore, the enzymes immobilized onto the PHB nanofibers exhibited markedly higher stability and activity compared to when immobilized on conventional immobilization supports. Our approach combines the advantageous features of nanofibrous material and specificity of biomolecular interaction for the efficient use of enzymes, which can be widely adopted in the development of various enzymatic processes.
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