Bioassociation of U(VI) and Eu(III) by Plant (Brassica napus) Suspension Cell Cultures.A Spectroscopic Investigation

In this study, we investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0-200 mu M) bioassociation experiments showed that more than 75% of U(VI) and Eu(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multistage bioassociation process for cells that were exposed to 200 mu M U(VI), where, after initial immobilization of U(VI) within 1 h of exposure, it was released back into the culture medium starting within 24 h. A remobilization to this extent has not been previously observed. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the remobilization of U back into the solution, which we assume to be a U(VI) phosphate species. Thus, the interaction of U(VI) and Eu(III) with released plant metabolites could be concluded.

Automated summary

This study investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells. Results showed that over 75% of U(VI) and Eu(III) were immobilized by the cells, with a multistage bioassociation process observed for cells exposed to 200μM U(VI). A new U species was identified, suggesting interaction with plant metabolites.