Enhanced reduction of lead bioavailability in phosphate mining wasteland soil by a phosphate-solubilizing strain of Pseudomonas sp., LA, coupled with ryegrass (Lolium perenne L.) and sonchus (Sonchus oleraceus L.)

Due to ecologically unsustainable mining strategies, there remain large areas of phosphate mining wasteland contaminated with accumulated lead (Pb). In this study, a Pb-resistant phosphate-solubilizing strain of Pseudomonas sp., LA, isolated from phosphate mining wasteland, was coupled with two species of native plants, ryegrass (Lolium perenne L.) and sonchus (Sonchus oleraceus L.), for use in enhancing the reduction of bioavailable Pb in soil from a phosphate mining wasteland. The effect of PbCO3 solubilization by Pseudomonas sp. strain LA was evaluated in solution culture. It was found that strain LA could attain the best solubilization effect on insoluble Pb when the PbCO3 concentration was 1% (w/v). Pot experiments were carried out to investigate the potential of remediation by ryegrass and sonchus in phosphate mining wastelands with phosphate rock application and phosphate-solubilizing bacteria inoculation. Compared to the control group without strain LA inoculation, the biomass and length of ryegrass and sonchus were markedly increased, available P and Pb in roots increased by 22.2%-325% and 23.3%-368%, respectively, and available P and Pb in above-ground parts increased by 4.44%-388% and 1.67%-303%, respectively, whereas available Pb in soil decreased by 14.1%-27.3%. These results suggest that the combination of strain LA and plants is a bioremediation strategy with considerable potential and could help solve the Pb-contamination problem in phosphate mining wastelands. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

The study demonstrated that the combination of Pb-resistant phosphate-solubilizing strain of Pseudomonas sp. LA with native plants ryegrass and sonchus could effectively reduce bioavailable Pb in phosphate mining wasteland soil. This bioremediation strategy showed significant potential in enhancing plant growth and mineral uptake, while reducing Pb contamination in the soil.