Assessing Indoor Dust Interference with Human Nuclear Hormone Receptors in Cell -Based Luciferase Reporter Assays

BACKGROUND: Per- and polyfluoroalkyl substances (PEAS), organophosphate esters (OPEs), and polybrominated diphenyl ethers (PBDEs) are hormone-disrupting chemicals that migrate from building materials into air and dust. OBJECTIVES: We aimed to quantify the hormonal activities of 46 dust samples and identify chemicals driving the observed activities. METHODS: We evaluated associations between hormonal activities of extracted dust in five cell-based luciferase reporter assays and dust concentrations of 42 measured PEAS, OPEs, and PBDEs, transformed as either raw or potency-weighted concentrations based on Tox21 high-throughput screening data. RESULTS: All dust samples were hormonally active, showing antagonistic activity toward peroxisome proliferator-activated receptor (PPAR72) (100%; 46 of 46 samples), thyroid hormone receptor (TAP) (89%; 41 samples), and androgen receptor (AR) (87%; 40 samples); agonist activity on estrogen receptor (ER alpha) (96%; 44 samples); and binding competition with thyroxine (T-4) on serum transporter transthyretin (TTR) (98%; 45 samples). Effects were observed with as little as 4 jig of extracted dust. in regression models for each chemical class, interquartile range increases in potency-weighted or unknown-potency chemical concentrations were associated with higher hormonal activities of dust extracts (potency-weighted: Sigma PFAS-TR beta, up arrow 28%, p < 0.05; Sigma OPEs-TR beta, up arrow 27%, p = 0.08; Sigma PBDEs-TR beta, up arrow 20%, p < 0.05; Sigma PBDEs-ER alpha, up arrow 17.7%, p = 0.08; unknown-potency: Sigma OPEs-TTR, up arrow 34%, p < 0.05; Sigma OPEs-AR, up arrow 13%, p = 0.06), adjusted for chemicals with active, inactive, and unknown Tox21 designations. DISCUSSION: All indoor dust samples exhibited hormonal activities, which were associated with PFAS, PBDE, and OPE levels.Reporter gene cell-based assays are relatively inexpensive, health-relevant evaluations of toxic loads of chemical mixtures that building occupants are exposed to.

Automated summary

The study found that all indoor dust samples exhibited hormonal activities, which were associated with PFAS, PBDE, and OPE levels, posing potential risks to human health.