Journal
ELECTROPHORESIS
Volume 42, Issue 20, Pages 1996-2009Publisher
WILEY
DOI: 10.1002/elps.202100028
Keywords
Daughter‐ cell dissections; Electrical impedance spectroscopy; Microelectrode array; Saccharomyces cerevisiae; Single‐ cell analysis
Funding
- National Natural Science Foundation of China [61774036, 31771299]
- National Key R&D Program of China [2018YFF01012100, 2018YFA0801100]
- Science and Technology Project of Jiangsu Province [BZ2020067]
- National Center for International Research [2017B01012]
- Fundamental Research Funds for the Central Universities
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
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This study reported on the three-dimensional finite-element modeling and simulations of impedance measurements for single yeast cell replicative lifespan, highlighting potential issues in impedance recording such as errors caused by daughter-cell dissections. Optimization of electrode array pitch was proposed to eliminate these errors, providing insights for the design and optimization of microfluidic electrode-array-integrated devices for high-throughput and accurate yeast RLS assays.
High-resolution microscopic imaging may cause intensive image processing and potential impact of light irradiation on yeast replicative lifespan (RLS). Electrical impedance spectroscopy (EIS) could be alternatively used to perform high-throughput and label-free yeast RLS assays. Prior to fabricating EIS-integrated microfluidic devices for yeast RLS determination, systematic modeling and theoretical investigation are crucial for device design and optimization. Here, we report three-dimensional (3D) finite-element modeling and simulations of EIS measurement in a microfluidic single yeast in-situ impedance array (SYIIA), which is designed by patterning an electrode matrix underneath a cell-trapping array. SYIIA was instantiated and modeled as a 5 x 5 sensing array comprising 25 units for cell immobilization, culturing, and time-lapse EIS recording. Simulations of yeast growing and budding in a sensing unit demonstrated that EIS signals enable the characterization of cell growth and daughter-cell dissections. In the 5 x 5 sensing array, simulation results indicated that when monitoring a target cell, daughter dissections in its surrounding traps may induce variations of the recorded EIS signals, which could cause mistakes in identifying target daughter-cell dissections. To eliminate the mis-identifications, electrode array pitch was optimized. Therefore, the results could conduct the design and optimization of microfluidic electrode-array-integrated devices for high-throughput and accurate yeast RLS assays.
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