A Sensitive Signal-off Electrochemical Aptasensor for Thrombin Detection using PB-Au@MoS2 Nanomaterial as Both Platform and Signal Reporter

Human serum is one of the effective samples for point-of-care testing (POCT). Sensitive and quick determination of thrombin content in human serum samples is important. An electrochemical aptasensor based on Prussian blue and Au nanoparticles loaded MoS2 nanoflowers (PB-Au@MoS2) hybrid was constructed. By using PB-Au@MoS2 as both a substrate and a signal reporter, this aptasensor could demonstrate excellent performance for thrombin detection with a detection linear range from 0.01 pM to 30 nM and detection limit down to 1 fM. This work may provide a strategy to establish effective and sensitive sensing devices for thrombin in clinical diagnosis.

Automated summary

The electrochemical aptasensor constructed based on PB-Au@MoS2 demonstrated excellent performance for thrombin detection, with a detection linear range from 0.01 pM to 30 nM and a detection limit down to 1 fM. This work may offer a strategy to develop effective and sensitive sensing devices for thrombin in clinical diagnosis.