Maternal nutrient restriction alters thyroid hormone dynamics in placentae of sheep having small for gestational age fetuses

Thyroid hormones regulate a multitude of metabolic and cellular processes involved in pla-cental and fetal growth, while maternal nutrient restriction (NR) has the potential to in-fluence these processes. Those fetuses most impacted by NR, as categorized by weight, are termed small for gestational age (SGA), but the role of thyroid hormones in these pregnan-cies is not fully understood. Therefore, the aims of the present study were to determine effects of NR during pregnancy on maternal and fetal thyroid hormone concentrations, as well as temporal and cell-specific expression of mRNAs and proteins for placental thyroid hormone transporters, thyroid hormone receptors, and deiodinases in ewes having either SGA or normal weight fetuses. Ewes with singleton pregnancies were fed either a 100% NRC (n = 8) or 50% NRC (NR; n = 28) diet from Days 35 to 135 of pregnancy with a single placentome surgically collected on Day 70. Fetal weight at necropsy on Day 135 was used to designate the fetuses as NR NonSGA (n = 7; heaviest NR fetuses) or NR SGA (n = 7; lightest NR fetuses). Thyroid hormone levels were lower in NR SGA compared to NR Non-SGA ewes, while all NR fetuses had lower concentrations of thyroxine at Day 135. Expres-sion of mRNAs for thyroid hormone transporters SLC16A2, SLC16A10, SLCO1C1, and SLCO4A1 were altered by day, but not nutrient restriction. Expression of THRA mRNA and protein was dysregulated in NR SGA fetuses with protein localized to syncytial and stromal cells in placentomes in all groups. The ratio of deiodinases DIO2 and DIO3 was greater for NR SGA placentae at Day 70, while DIO3 protein was less abundant in placentae from NR SGA than 100% NRC ewes. These results identify mid-gestational modifications in thyroid hormone-associated proteins in placentomes of ewes having SGA fetuses, as well as a potential for placentomes from NonSGA pregnancies to adapt to, and overcome, nutritional restrictions during pregnancy. (c) 2021 Elsevier Inc. All rights reserved.

Automated summary

Maternal nutrient restriction during pregnancy can affect fetal growth and thyroid hormone levels, leading to lower thyroid hormone concentrations in NR SGA fetuses. Changes in thyroid hormone-associated proteins in placentomes of ewes having SGA fetuses suggest adaptations to and potential effects of nutritional restrictions.