Neuronal Apoptosis Preventive Potential of Sophocarpine via Suppression of Aβ-Accumulation and Down-Regulation of Inflammatory Response

In the current study sophocarpine was investigated in vitro for prevention of beta-amyloid induced PC12 neuronal cell damage. Exposure to beta-amyloid caused a dose-dependent suppression in growth of PC12 cells with maximum reduction at 10 mu M. Sophocarpine pre-treatment reversed suppressive effect of beta-amyloid (10 mu M) on PC12 cell growth in concentration-based manner. In sophocarpine pre-treated PC12 cells the beta-amyloid mediated PGE2 level elevation was attenuated significantly at 0.25-2 mu M doses. Moreover, in sophocarpine pretreated PC12 cells the beta-amyloid mediated promotion of COX-2 level was also inhibited. Sophocarpine pre-treatment attenuated iNOS expression in beta-amyloid exposed PC12 cells at 0.25-2 mu M doses. Pre-treatment of PC12 cells with sophocarpine suppressed NO-species generation induced by beta-amyloid exposure. In sophocarpine pretreated PC12 cells elevation of nuclear NF-kappa B expression induced by beta-amyloid was significantly inhibited. In summary, sophocarpine prevents reduction of PC12 cell growth induced by beta-amyloid exposure via inhibition of inflammatory processes. The preventive effect of sophocarpine on beta-amyloid induced PC12 cell damage is associated with inhibition of NF-kappa B nuclear translocation. Therefore, sophocarpine may be used for treatment of neurological disorders like Alzheimer's disease.

Automated summary

Sophocarpine prevents reduction of PC12 cell growth induced by beta-amyloid exposure via inhibition of inflammatory processes. The preventive effect of sophocarpine on beta-amyloid induced PC12 cell damage is associated with inhibition of NF-kappa B nuclear translocation, making it a potential treatment for neurological disorders like Alzheimer's disease.