Journal
DEVELOPMENT
Volume 148, Issue 9, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.182667
Keywords
CRISPR screening; CRISPR-Cas9; Disease modeling; Epigenetics; Gene regulation
Categories
Funding
- National Institutes of Health [R01HG008754, R21HG010391]
- American Cancer Society
- American Heart Association
- National Organization for Rare Disorders
- Qatar Biomedical Research Institute
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Merkin Institute for Transformative Technologies in Healthcare
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Understanding how genes are expressed correctly in cells is a key goal of developmental biology research, with CRISPR-Cas9 technology transforming the study of gene regulation through precise manipulation of genomic sequences and expression, leading to the discovery of new paradigms in gene regulation and potentially revolutionizing our ability to manipulate cell fate.
Understanding how genes are expressed in the correct cell types and at the correct level is a key goal of developmental biology research. Gene regulation has traditionally been approached largely through observational methods, whereas perturbational approaches have lacked precision. CRISPR-Cas9 has begun to transform the study of gene regulation, allowing for precise manipulation of genomic sequences, epigenetic functionalization and gene expression. CRISPR-Cas9 technology has already led to the discovery of new paradigms in gene regulation and, as new CRISPR-based tools and methods continue to be developed, promises to transform our knowledge of the gene regulatory code and our ability to manipulate cell fate. Here, we discuss the current and future application of the emerging CRISPR toolbox toward predicting gene regulatory network behavior, improving stem cell disease modeling, dissecting the epigenetic code, reprogramming cell fate and treating diseases of gene dysregulation.
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