Journal
DEVELOPMENT
Volume 148, Issue 18, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.198507
Keywords
3D reconstruction; Actin; Development; Image processing; Nucleus
Categories
Funding
- Japan Society for the Promotion of Science KAKENHI [18H02450, 15H05857]
- Grants-in-Aid for Scientific Research [15H05857, 18H02450] Funding Source: KAKEN
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Proper positioning of nuclei in the Drosophila anterior midgut plays a crucial role in subsequent LR-asymmetric development of the organ. Wnt4 signaling, myosin II, and the LING complex are important for collective nuclear behavior and proper positioning.
Proper organ development often requires nuclei to move to a specific position within the cell. To determine how nuclear positioning affects left-right (LR) development in the Drosophila anterior midgut (AMG), we developed a surface-modeling method to measure and describe nuclear behavior at stages 13-14, captured in three-dimensional time-lapse movies. We describe the distinctive positioning and a novel collective nuclear behavior by which nuclei align LR symmetrically along the anterior-posterior axis in the visceral muscles that overlie the midgut and are responsible for the LR-asymmetric development of this organ. Wnt4 signaling is crucial for the collective behavior and proper positioning of the nuclei, as are myosin II and the LING complex, without which the nuclei fad to align LR symmetrically. The LR-symmetric positioning of the nuclei is important for the subsequent LR-asymmetric development of the AMG. We propose that the bilaterally symmetrical positioning of these nuclei may be mechanically coupled with subsequent LR-asymmetric morphogenesis.
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