Formin-mediated bridging of cell wall, plasma membrane, and cytoskeleton in symbiotic infections of Medicago truncatula

Legumes have maintained the ability to associate with rhizobia to sustain the nitrogen-fixing root nodule symbiosis (RNS). In Medicago truncatula, the Nod factor (NF)-dependent intracellular root colonization by Sinorhizobium meliloti initiates from young, growing root hairs. They form rhizobial traps by physically curling around the symbiont.(1,2) Although alterations in root hair morphology like branching and swelling have been observed in other plants in response to drug treatments(3) or genetic perturbations,(4-6) full root hair curling represents a rather specific invention in legumes. The entrapment of the symbiont completes with its full enclosure in a structure called the infection chamber'' (IC),(1,2,7,8) from which a tubelike membrane channel, the infection thread'' (IT), initiates.(1,2,9) All steps of rhizobium-induced root hair alterations are aided by a tip-localized cytosolic calcium gradient,(10,11) global actin re-arrangements, and dense subapical fine actin bundles that are required for the delivery of Golgi-derived vesicles to the root hair tip.(7,12-14) Altered actin dynamics during early responses to NFs or rhizobia have mostly been shown in mutants that are affected in the actin-related SCAR/WAVE complex.(15-18) Here, we identified a polarly localized SYMBIOTIC FORMIN 1 (SYFO1) to be required for NF-dependent alterations in membrane organization and symbiotic root hair responses. We demonstrate that SYFO1 mediates a continuum between the plasma membrane and the cell wall that is required for the onset of rhizobial infections.

Automated summary

Legumes have the ability to associate with rhizobia for nitrogen-fixing root nodule symbiosis. In Medicago truncatula, root hair colonization by Sinorhizobium meliloti begins from young root hairs, which physically entwine around the symbiont to form rhizobial traps. This unique process involves alterations in membrane organization, cytosolic calcium gradient, actin rearrangements, and symbiotic responses in legumes.