Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
Volume 243, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2021.108982
Keywords
Lipopolysaccharide; Intestinal inflammation; Tight junctions; Vitamin D-3; Pelteobagrus fulvidraco
Funding
- Zhejiang Provincial Natural Science Foundation of China [LY19D060001]
- K.C. Wong Magna Fund at Ningbo University
Ask authors/readers for more resources
The study revealed that exogenous VD3 substantially alleviates LPS-induced intestinal inflammation by upregulating antioxidant activity, suppressing inflammation, and promoting the expression of fencing tight junction genes in the intestine, while having no effect on apoptosis genes.
The present study explored the possible mitigative effects of vitamin D-3 (VD3) on lipopolysaccharide (LPS)-induced intestinal oxidative stress, inflammatory response and tight junction damage in yellow catfish, Pelteobagrus fulvidraco. Herein, four experimental groups were established by injecting yellow catfish with NaCl, LPS, VD3 or LPS plus VD3. The results showed that LPS induced oxidative stress and that exogenous VD3 mitigated the adverse effects of LPS. Additionally, LPS suppressed the activity of antioxidant enzymes (Cat, Sod and Gr) and upregulated the mRNA expression of proinflammatory cytokines (Tnf-alpha, Il-1 beta, Il-8). Furthermore, the mRNA expression of fencing tight junctions (Claudin-1, Claudin-5, Occludin, Zo-1) was downregulated, while that of pore-forming tight junctions (Claudin-2, Claudin-12) was upregulated, however no effect on apoptosis genes was observed (p53, Bax, Caspase-3 and Caspase-9). These LPS-induced effects were significantly reversed by pretreatment with VD3. Taken together, this study suggests that exogenous VD3 substantially alleviates LPS-induced intestinal inflammation by upregulating antioxidant activity, suppressing inflammation and promoting fencing tight junctions in the intestine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available