Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells

Objective To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted. The nitric oxide (NO) production was measured using the Griess assay. Prostaglandin E-2 (PGE(2)) production was evaluated with enzyme-linked immunosorbent assay. The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis. Furthermore, phosphorylation of nuclear factor-kappa B (NF-kappa B) and nuclear translocation of NF-kappa B p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively. Results Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE(2) in LPS-stimulated RAW 264.7 cells (PP<0.05). Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01). Phosphorylation of NF-kappa B and nuclear translocation of NF-kappa B p65 was significantly inhibited by BNH (PP<0.05). Conclusion The anti-inflammatory activities of BNH were mediated via blockage of the NF-kappa B signaling pathways in LPS-stimulated RAW 264.7 cells.

Automated summary

The experiment demonstrated that Brassica napus L. hydrosols exhibit significant anti-inflammatory activity in LPS-stimulated RAW 264.7 cells by reducing the generation of nitric oxide and prostaglandin E-2, inhibiting the expression of inducible nitric oxide synthase and cyclooxygenase-2, and blocking the NF-kappa B signaling pathways.