Naive stem cell blastocyst model captures human embryo lineage segregation

Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.

Automated summary

Researchers have developed a human embryo model called blastoid through self-organization, which can form blastocyst-like structures within a short period of time, displaying three tissue layers and mimicking natural blastocysts. Single-cell transcriptome analysis confirms the high fidelity of the blastoid model, providing a versatile and scalable experimental model for human embryo research.