Journal
CELL BIOLOGY INTERNATIONAL
Volume 45, Issue 7, Pages 1498-1509Publisher
WILEY
DOI: 10.1002/cbin.11589
Keywords
cytokines; microRNAs; multiple sclerosis; oxygen– ozone therapy; transcription factor; Treg cell
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Funding
- Baqiyatallah University of Medical Sciences
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Oxygen-ozone therapy can improve the condition of multiple sclerosis patients by increasing the frequency of Treg cells and the expression levels of related factors, decreasing the expression level of miR-146a, and significantly increasing the secretion levels of IL-10 and TGF-beta cytokines.
Multiple sclerosis (MS) is a common degenerative disorder of the central nervous system. The decreased frequency and dysfunction of Treg cells cause inflammation and disease progression. Ozone autohemotherapy can be used as a potential therapeutic approach to regulate the immune system responses and inflammation in MS. For this purpose, 20 relapsing-remitting multiple sclerosis patients were under treatment with ozone twice weekly for 6 months. The frequency of Treg cell, the expression levels of the Treg cell-related factors (FoxP3, IL-10, TGF-beta, miR-17, miR-27, and miR-146A), and the secretion levels of IL-10 and TGF-beta were assessed. We found a significant increase in the number of Treg cells, expression levels of FoxP3, miRNAs (miR-17 and miR-27), IL-10, and TGF-beta factors in patients after oxygen-ozone (O-2-O-3) therapy compared to before treatment. In contrast, oxygen-ozone therapy notably decreased the expression level of miR-146a in treated patients. Interestingly, the secretion levels of both IL-10 and TGF-beta cytokines were considerably increased in both serum and supernatant of cultured peripheral blood mononuclear cells in posttreatment condition compared to pretreatment condition. According to results, oxygen-ozone therapy raised the frequency of Treg cell and its relevant factors in treated MS patients. Oxygen-ozone therapy would contribute to improving the MS patients by elevating the Treg cell responses.
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