Journal
CARBOHYDRATE POLYMERS
Volume 258, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.117720
Keywords
Chitin; Successive co-fermentation; Acetobacter pasteurianus; Deproteinization; Demineralization
Categories
Funding
- key Science and Technology Project of Hainan Province, China [ZDYF2019138]
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In this study, a process of chitin bio-extraction from shrimp shells powder was simplified by using successive co-fermentation with Bacillus subtilis and Acetobacter pasteurianus. The purified chitin obtained from this method showed excellent structural characteristics compared to commercial chitin, with high deacetylation degree and lower molecular weight.
To simplify the process of chitin bio-extraction from shrimp shells powder (SSP), successive co-fermentation using Bacillus subtilis and Acetobacter pasteurianus was explored in this work. Among three protease-producer (B. licheniformis, B. subtilis, and B. cereus), only B. subtilis exhibited high compatibility with A. pasteurianus in co-culture. Successive co-fermentation was constructed as follows: deproteinization was performed for 3 d by culturing B. subtilis in the medium containing 50 g.L-1 SSP, 50 g.L-1 glucose, and 1 g.L-1 yeast extracts; After feeding 5 g.L-1 KH2PO4 and 6 % (v/v) ethanol, A. pasteurianus was cultured for another 2 d without replacing and re-sterilizing medium. Through 5 d of fermentation, the final deproteinization, demineralization efficiency, and chitin yield reached 94.5 %, 92.0 %, and 18.0 %, respectively. This purified chitin had lower molecular weight (12.8 kDa) and higher deacetylation degree (19.6 %) compared with commercial chitin (18.5 kDa, 6.7 %), and showed excellent structural characterization of FESEM and FT-IR analysis.
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