Journal
CARBOHYDRATE POLYMERS
Volume 257, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117605
Keywords
Purple sweet potato; NMR spectrometry; Scanning electron microscope; Atomic force microscopy; Circular dichroism; Microstructure
Categories
Funding
- Natural Science Foundation of China [81770463, 82070469]
- Natural Science Foundation of Heilongjiang Province [LH2020H071, H2015088]
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A novel glucan PSPP-1 was purified from the foot tuber of purple sweet potato, with a backbone composed of alpha-D-Glcp residues and branching at different positions with alpha-D-Glcp residues. Various experiments revealed its amorphous form, microstructure, particle size, and inhibitory activities on different cells. The findings contribute to understanding the structural characteristics and potential applications of sweet potato polysaccharides.
A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam. Its backbone was composed of -> 4)-alpha-D-Glcp(1 -> glycosyl, and branching at the O-2, O-3, and O-6 positions with alpha-D-Glcp(1 -> residues. X-ray diffraction experiment showed that PSPP-1 existed as an amorphous form. Its microstructure was detected via scanning electron microscopy. Its particle size was mainly concentrated at 230 nm in water. Congo red and circular dichroism experiments showed there was no triple-helix conformation. Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was-800 nm and-7.0 mu m, respectively. Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells. Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.
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