Journal
CANCER RESEARCH
Volume 81, Issue 9, Pages 2318-2331Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-20-2331
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Funding
- Japan Society for the Promotion of Science [16K08623, 23112508, 26460389, 17K08627, 20K08489]
- Shiga University of Medical Science In-House Grant
- Uehara Memorial Foundation
- Takeda Science Foundation
- Naito Foundation
- Grants-in-Aid for Scientific Research [20K08489, 16K08623, 17K08627] Funding Source: KAKEN
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Stromal-induced stomatin expression in prostate cancer cells suppresses tumor growth by inhibiting the Akt signaling axis, with clinical relevance shown in decreased stomatin levels in high Gleason score prostate cancer samples and higher recurrence rates post-operation.
The growth and progression of cancers are crucially regulated by the tumor microenvironment where tumor cells and stromal cells are mutually associated. In this study, we found that stomatin expression was markedly upregulated by the interaction between prostate cancer cells and stromal cells. Stomatin suppressed cancer cell proliferation and enhanced apoptosis in vitro and inhibited xenograft tumor growth in vivo. Stomatin inhibited Akt activation, which is mediated by phosphoinositide-dependent protein kinase 1 (PDPK1). PDPK1 protein stability was maintained by its binding to HSP90. Stomatin interacted with PDPK1 and interfered with the PDPK1-HSP90 complex formation, resulting in decreased PDPK1 expression. Knockdown of stomatin in cancer cells elevated Akt activation and promoted cell increase by promoting the interaction between PDPK1 and HSP90. Clinically, stomatin expression levels were significantly decreased in human prostate cancer samples with high Gleason scores, and lower expression of stomatin was associated with higher recurrence of prostate cancer after the operation. Collectively, these findings demonstrate the tumor-suppressive effect of stromal-induced stomatin on cancer cells. Significance: These findings reveal that interactions with stromal cells induce expression of stomatin in prostate cancer cells, which suppresses tumor growth via attenuation of the Akt signaling axis.
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