Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

A12-week experiment was conducted to evaluate the influences of thiamine ongrowth performance, and intestinal mitochondrial biogenesis and function of Megalobramaamblycephala fed a high-carbohydrate (HC) diet. Fish (24 center dot 73 (sem 0 center dot 45) g) were randomly assigned to one of four diets: two carbohydrate (CHO) levels (30 and 45 %) and two thiamine levels (0 and 1 center dot 5 mg/kg). HC diets significantly decreased DGC, GR(MBW), FIMBW, intestinal activities of amylase, lipase, Na+, K+-ATPase, CK, complexes I, III and IV, intestinal ML, number of mitochondrial per field, Delta psi m, the P-AMPK: T-AMPK ratio, PGC-1 beta protein expression as well as the transcriptions of AMPK alpha 1, AMPK alpha 2, PGC-1 beta, mitochondrial transcription factor A, Opa-1, ND-1 and COX-1 and 2, while the opposite was true for ATP, AMP and reactive oxygen species, and the transcriptions of dynamin-related protein-1, fission-1 and mitochondrial fission factor. Dietarythiamine concentrations significantly increased DGC, GR(MBW), intestinal activities of amylase, Na+, K+-ATPase, CK, complexes I and IV, intestinal ML, number of mitochondrial per field, Delta psi m, the P-AMPK:T-AMPK ratio, PGC-1 beta protein expression as well as the transcriptions of AMPK alpha 1, AMPK alpha 2, PGC-1 beta, Opa-1, ND-1, COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between dietary CHO and thiamine was observed in DGC, GR(MBW), intestinal activities of amylase, CK, complexes I and IV, Delta psi m, the AMP:ATP ratio, the P-AMPK:T-AMPK ratio, PGC-1 beta protein expression as well as the transcriptions of AMPK alpha 1, AMPK alpha 2, PGC-1 beta, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation improved growth performance, and intestinal mitochondrial biogenesis and function of M. amblycephala fed HC diets.

Automated summary

This study evaluated the influences of thiamine on growth performance and intestinal mitochondrial biogenesis and function of Megalobramaamblycephala fed a high-carbohydrate diet. The results showed that thiamine supplementation improved growth performance and intestinal mitochondrial biogenesis and function of the fish.