Journal
BRIEFINGS IN FUNCTIONAL GENOMICS
Volume 21, Issue 1, Pages 4-12Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bfgp/elab011
Keywords
ageing; budding yeast; fission yeast; chronological; phenomics; TOR
Funding
- UEL
Ask authors/readers for more resources
Cellular models such as yeasts, which have simpler genomes, shorter lifespans and abundant genetic resources, play an important role in biogerontology research. Yeasts have contributed to the understanding of fundamental aspects of lifespan regulation, including nutrient response, protein translation, DNA damage, oxidative stress, mitochondrial function and autophagy. The use of automation and computational tools has allowed for the manipulation and analysis of genome-wide lifespan data, leading to a comprehensive view of cellular ageing.
Cellular models such as yeasts are a driving force in biogerontology studies. Their simpler genome, short lifespans and vast genetic and genomics resources make them ideal to characterise pro-ageing and anti-ageing genes and signalling pathways. Over the last three decades, yeasts have contributed to the understanding of fundamental aspects of lifespan regulation including the roles of nutrient response, global protein translation rates and quality, DNA damage, oxidative stress, mitochondrial function and dysfunction as well as autophagy. In this short review, we focus on approaches used for competitive and non-competitive cell-based screens using the budding yeast Saccharomyces cerevisiae, and the fission yeast Schizosaccharomyces pombe, for deciphering the molecular mechanisms underlying chronological ageing. Automation accompanied with appropriate computational tools allowed manipulation of hundreds of thousands of colonies, generation, processing and analysis of genome-wide lifespan data. Together with barcoding and modern mutagenesis technologies, these approaches have allowed to take decisive steps towards a global, comprehensive view of cellular ageing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available