Journal
BIOSENSORS & BIOELECTRONICS
Volume 178, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113024
Keywords
Electrochemiluminescence; Carbohydrate antigen 24-2; Carbohydrate antigen 19-9; Immunosensor; Metal-organic framework
Categories
Funding
- National Natural Science Foundation of China [21765004]
- Guangxi Science Foundation of China [2019GXNSFAA245076]
- Innovation Project of Guangxi Graduate Education [XYCBZ2020001]
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) [CMEMR2018-C18]
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A new ECL immunosensor based on spatially-resolved dual-potential technology was developed for simultaneous detection of CA 19-9 and CA 242, showing high selectivity and stability in real sample analysis.
A new electrochemiluminescence (ECL) immunosensor based on spatially-resolved dual-potential technology was designed for the simultaneous determination of carbohydrate antigen 19-9 (CA 19-9) and carbohydrate antigen 24-2 (CA 242). Luminol-AgNPs@ZIF-67 was used as the anodic probe, and Pt nanoparticle-functionalized graphitic carbon nitride nanosheets (g-C3N4@PtNPs) were used as the cathodic probe. Two spatially-resolved areas on the dual-disk glassy carbon electrode (DDGCE) were modified with a AuNPs film by electrodeposition to improve the conductivity of the sensing interface. By recording the ECL responses at two different excitation potentials, the linear range for CA 19-9 was determined to be 0.0001-10 U/mL, with a limit of detection of 31 mu U/mL. The linear range for CA 242 was 0.0005-10 U/mL, with a limit of detection of 0.16 mU/mL. Moreover, the ECL immunosensor possessed high selectivity and stability and successfully detected CA 19-9 and CA 242 in real samples. This immunosensor provides a new platform for clinical immunoassays.
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