Journal
BIOSENSORS & BIOELECTRONICS
Volume 177, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.112977
Keywords
Tetanus toxoid; Tetanus human IgG-AuNCs; Fluorescence; Immunochromatography test strip
Categories
Funding
- National Natural Science Foundation of China [21675024, 21804021]
- Joint Funds for the Innovation of Science and Technology, Fujian Province [2019Y9007]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Science and Technology Project of Fujian Province [2018L3008]
Ask authors/readers for more resources
The development of Tet-IgG-AuNCs through a facile biomineralization process for detecting tetanus toxoid has shown promising results. The immunochromatography test strip utilizing fluorescent Tet-IgG-AuNCs demonstrated good sensitivity with a detection limit of 0.03μg/mL.
Assays for detecting tetanus toxoid are of great significance to be applied in the research of the safety testing of tetanus vaccine. Currently, guinea pigs or mice are usually used to evaluate the toxicity in these assays. Herein, a facile and quick biomineralization process was carried out to generate tetanus human immunoglobulin G (TetIgG)-functionalized Au nanoclusters (Tet-IgG-AuNCs). The obtained Tet-IgG-AuNCs exhibited strong red emission with a photoluminescence quantum yield of 13%. Based on surface plasmon resonance measurements, the apparent dissociation constant of the Tet-IgG-AuNC-tetanus toxoid complexes was measured to be 2.27 x 10(-8) M. A facile detection approach was developed using a fluorescent Tet-IgG-AuNC-based immunochromatography test strip. By utilizing the high-brightness fluorescent Tet-IgG-AuNCs, this immunosensor showed favorable sensitivity with a detection limit at the level of 0.03 mu g/mL. Further results demonstrated that this assay can reliably detect tetanus toxoid and therefore might provide a novel method to replace animal tests for the quantification of tetanus toxicity. Moreover, the antibody-AuNC-based immunochromatography test strip platform serves as a promising candidate to develop new approaches for detecting targeted antigens and biological events of interest.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available