Degradation of ofloxacin by a manganese-oxidizing bacterium Pseudomonas sp. F2 and its biogenic manganese oxides

Fluoroquinolone antibiotics like ofloxacin (OFL) have been frequently detected in the aquatic environment. Recently manganese-oxidizing bacteria (MOB) have attracted research efforts on the degradation of recalcitrant pollutants with the aid of their biogenic manganese oxides (BioMnOx). Herein, the degradation of OFL with a strain of MOB (Pseudomonas sp. F2) was investigated for the first time. It was found that the bacteria can degrade up to 100% of 5 ?g/L OFL. BioMnOx and Mn(III) intermediates significantly contributed to the degradation. Moreover, the degradation was clearly declined when the microbial activity was inactivated by heat or ethanol, indicating the importance of bioactivity. Possible transformation products of OFL were identified by HPLC-MS and the degradation pathway was proposed. In addition, the toxicity of OFL was reduced by 66% after the degradation.

Automated summary

This study for the first time demonstrated that manganese-oxidizing bacteria Pseudomonas sp. F2 can effectively degrade OFL in water environments, with the degradation relying on biogenic manganese oxides and Mn(III) intermediates. Additionally, the decreased degradation when microbial activity was inactivated highlights the importance of bioactivity in the degradation process.