Effects of operating conditions on microbial consortium of the heterotrophic ammonia oxidation process

This study aimed to investigate the ammonia removal by the consortium mainly comprising of ammonia-oxidizing bacteria under different initial pH, temperatures and stress of heavy metals. The results showed that the consortium exhibited a strong adaptation for broad pH ranging from 5 to 9. When the temperature dropped to 15 degrees C, its ammonium removal and nitrate accumulation rates decreased by 72.23% and 95.12%, respectively. Meanwhile, the temperature correction coefficients of the ammonium removal and nitrate accumulation rates reached the maximum. In addition, the consortium could survive in the solutions containing 0-1.0 mg.L-1 Cu2+ and 0-5.0 mg.L-1 Fe3+. Moreover, the inhibition of free nitrous acid (FNA) against nitrite oxidation activity was found to be much more significant than that low-temperature treatment. Microbial diversity analysis showed that the bacterial community structure was shift significantly by the temperature drop, especially change the abundance of Nitrosomonas, Paracoccus, Pseudomonas and Nitrospirae.

Automated summary

The study found that the consortium of ammonia-oxidizing bacteria has a wide pH tolerance range and can adapt well under acidic and alkaline conditions. With a decrease in temperature, the rates of ammonium removal and nitrate accumulation are significantly reduced. Additionally, the consortium can survive in solutions containing copper ions and iron ions.