Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform

Isatoic anhydride (IA) has been shown to be a useful platform for quantifiable bioconjugation. The elaboration of a water-soluble isatoic anhydride-based platform with biotin offers readily quantifiable biotinylation reagents through nondestructive methods of quantification. The incorporation of functionality is directly quantified using the reagent's unique absorbance or fluorescence signature, located outside the biological window. Several biotinylation reagents are prepared with various linker lengths, and the quantification of biotinylated proteins is demonstrated and compared to results from the traditional HABA assay.

Automated summary

Isatoic anhydride serves as a useful platform for quantifiable bioconjugation, allowing for the preparation of quantifiable biotinylation reagents through nondestructive methods. The incorporation of functionality is directly quantified using unique absorbance or fluorescence signatures, demonstrating successful quantification of biotinylated proteins compared to traditional methods.