Journal
BIOCHEMICAL SOCIETY TRANSACTIONS
Volume 49, Issue 3, Pages 1233-1240Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BST20200833
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Funding
- Wellcome Trust Strategic Award [107457]
- Wellcome Trust Senior Investigator Award [104575]
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The new SAP model offers a novel approach to study interactions of core centriole proteins in the absence of authentic centrioles. While there are strengths in this model, further validation and discussions are needed in the context of other models for centriole study.
Centrioles are important cellular organelles involved in the formation of both cilia and centrosomes. It is therefore not surprising that their dysfunction may lead to a variety of human pathologies. Studies have identified a conserved pathway of proteins required for centriole formation, and investigations using the embryo of the fruit fly Drosophila melanogaster have been crucial in elucidating their dynamics. However, a full understanding of how these components interact has been hampered by the total absence of centrioles in null mutant backgrounds for any of these core centriole factors. Here, I review our recent work describing a new model for investigating these interactions in the absence of bona fide centrioles. Sas-6 Ana2 Particles (SAPs) form when two core centriole factors, Sas-6 and Ana2, are co-over-expressed in fruit fly eggs. Crucially, they form even in eggs lacking other core centriole proteins. I review our characterisation of SAPs, and provide one example of how they have been used to investigate the role of a core centriole protein in PCM formation. I then consider some of the strengths and weaknesses of the SAP model, and discuss them in the context of other models for centriole study in Drosophila. Similar aggregates have been seen in other systems upon expression of centriole factors, so SAPs may also be a useful approach to study centriole proteins in other organisms.
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