Journal
AUTOPHAGY
Volume 17, Issue 5, Pages 1281-1283Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2021.1909410
Keywords
Selective autophagy receptors; APEX2; proximity proteomics; proteostasis challenges; endosomal microautophagy; TOLLIP
Categories
Funding
- Deutsche Forschungsgemeinschaft [DFG, German Research Foundation] [390857198, 259130777]
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Selective autophagy receptors play a role in degrading cellular components of different sizes and rigidity. By combining various techniques, the study identified new autophagy substrates and found that some substrates are degraded through endosomal microautophagy instead of canonical autophagy pathways.
Selective autophagy receptors have been implicated in the degradation of cellular constituents of various size and rigidity. However, the identity of protein cargo have largely remained elusive. In our recent study, we combined limited proteolysis-enhanced proximity biotinylation and organelle enrichment with quantitative proteomics to map the inventory of autophagosomes in a manner dependent on six different selective autophagy receptors, namely SQSTM1/p62, NBR1, CALCOCO2/NDP52, OPTN, TAX1BP1 and TOLLIP. Conducting this approach under basal and proteostasis-challenged conditions in mammalian cells led to the identification of various new autophagy substrates of which some were degraded through endosomal microautophagy rather than canonical autophagy dependent on the receptors TOLLIP and SQSTM1, respectively.
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