Journal
ATHEROSCLEROSIS
Volume 330, Issue -, Pages 85-94Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.atherosclerosis.2021.04.003
Keywords
Single cell RNA sequencing; CyTOF; CITE-Seq; Atherosclerosis; Immune cells
Funding
- NIH-NHLBI [R01 HL153712-01]
- NIH-NCATS [UH3TR002067]
- AHA [20SFRN35210252]
- CZI [NFL-2020-218415]
- NIH [T32HL007824]
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The accumulation of lipids in the arterial wall triggers a network of inflammatory processes, contributing to the development of atherosclerosis. Recent clinical findings suggest that targeting specific immune alterations can prevent recurrent cardiovascular events. Single-cell approaches like CyTOF, scRNA-seq, and CITE-seq have emerged as valuable tools in understanding cellular plasticity within atherosclerotic lesions, but challenges remain in applying these methods to human vascular tissue.
Atherosclerosis is initiated by the accumulation of lipids in the arterial wall that trigger a complex and poorly understood network of inflammatory processes. At the same time, recent clinical findings reveal that targeting specific immune alterations in patients with cardiovascular disease (CVD) represents a promising approach to preventing recurrent cardiovascular events. In order to achieve these tailored therapies, it is critical to resolve the heterogenous environment of the atherosclerotic lesion and decipher the complex structural and functional changes which immune cells undergo throughout disease progression. Recently, single-cell approaches including single cell mass cytometry by time of flight (CyTOF), single cell RNA sequencing (scRNA-seq) and Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) have emerged as valuable tools in resolving cellular plasticity within atherosclerotic lesions. In this review, we will discuss the most important insights that have been gleaned from the application of these single-cell approaches to validated experimental models of atherosclerosis. Additionally, as clinical progress in treatment of the disease depends on the translation of discoveries to human tissues, we will also examine the challenges associated with the application of single-cell approaches to human vascular tissue and the discoveries made by the initial efforts in this direction. Finally, we will analyze the advantages and limitations of dissociative single-cell approaches and how novel in-situ technologies could advance the field by allowing for the investigation of individual cells while preserving the heterogenous architecture of the atherosclerotic lesion.
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