Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 89, Issue -, Pages 31-38Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2016.03.001
Keywords
Nanofibers; Laccase immobilization; Bisphenol A; 17 alpha-ethinylestradiol
Categories
Funding
- 'Environmental Friendly Nanotechnologies and Biotechnologies in Water and Soil Treatment Project' (NanoBioWat) [TE01020218]
- National Programme for Sustainability I
- 'OPR&DI Project' of the Centre for Nanomaterials, Advanced Technologies and Innovation [CZ.1.05/2.1.00/01.0005]
- Ministry of Education of the Czech Republic under 'SGS' Project via the Technical University of Liberec [21066/115]
- MICINN [JCI-2012-12705]
- MINECO [SAF2014-52632-R]
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In recent years, there has been an increase in efforts to improve wastewater treatment as the concentration of dangerous pollutants, such as endocrine disrupting chemicals, in wastewater increases. These compounds, which mimic the effect of hormones, have a negative impact on human health and are not easily removed from water. One way to effectively eliminate these pollutants is to use enzymatically activated materials. In this study, we report on the use of laccase from the white rot fungus Trametes versicolor immobilized onto polyamide 6/chitosan (PA6/CHIT)nanofibers modified using two different spacers (bovine serum albumin and hexamethylenediamine). We then tested the ability of the PA6/CHIT-laccase biocatalysts to eliminate a mixture containing 50 mu M of two endocrine disrupting chemicals: bisphenol A and 17 alpha-ethinylestradiol. The PA6/CHIT nanofiber matrix used in this study not only proved to be a suitable carrier for immobilized and modified laccase but was also efficient in the removal of a mixture of endocrine disrupting chemicals in three treatment cycles. (C) 2016 Elsevier Inc. All rights reserved.
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