Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 82, Issue -, Pages 8-14Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2015.08.012
Keywords
Cellulase; Trichoderma reesei; Phosphorus; Nitrogen; Pellet
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Funding
- U.S. Department of Agriculture under the Biomass Research and Development Initiative [2009-10001-05112]
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This work describes the use of nutrient limitations with Trichoderma reesei Rut C-30 to obtain a prolonged stationary phase cellulase production. This period of non-growth may allow for dependable cellulase production, extended fermentation periods, and the possibility to use pellet morphology for easy product separation. Phosphorus limitation was successful in halting growth and had a corresponding specific cellulase production of 5 +/- 2 FPU/g-h. Combined with the addition of Triton X-100 for fungal pellet formation and low shear conditions, a stationary phase cellulase production period in excess of 300h was achieved, with a constant enzyme production rate of 7 1 FPU/g-h. While nitrogen limitation was also effective as a growth limiter, it, however, also prevented cellulase production. (C) 2015 Elsevier Inc. All rights reserved.
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