Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa

High-throughput sequencing (HTS) was used to construct the virome profile of an old grapevine-leafroll-diseased grapevine (Vitis vinifera). De novo assembly of HTS data showed a complex infection, including a virus sequence with similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 7090 nucleotides and has four open reading frames (ORFs). Genome organisation and phylogenetic analysis identify this virus as a divergent variant of grapevine Roditis leaf discoloration-associated virus (GRLDaV) with 90% nucleotide sequence identity to isolate w4 (NC_027131). This is the first genome sequence of a South African variant of GRLDaV.

Automated summary

High-throughput sequencing was utilized to analyze the virome profile of a grapevine infected with grapevine leafroll disease, revealing a virus sequence similar to those of the genus Badnavirus. The complete genome sequence of this virus, a divergent variant of grapevine Roditis leaf discoloration-associated virus (GRLDaV), was identified with 90% nucleotide sequence identity to an isolate from South African variant.